Part:BBa_K594008:Design
a luxR-type regulator from Rhizobium etli CFN42
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 248
Illegal NgoMIV site found at 344
Illegal NgoMIV site found at 508 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Rhizobium etli CFN42 is also a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in Rhizobium etli CFN42, and then found the specific sequence in NCBI and asked the Rhizobium etli CFN42 from Nanjing Agricultural University. The raiI/R system have no threats to biosafety.
Source
We got the Rhizobium etli CFN42 from Nanjing Agriculture University, and then cloned the raiR gene from Rhizobium etli CFN42 with primers designed by the part design tool in the website [ginkgobioworks.com]. We get its gene sequence from the www.microbesonline.org, and then cloned them through PCR with biobrick part primers.
References
1.VIOLA ROSEMEYER et al.luxI- and luxR-Homologous Genes of Rhizobium etli CNPAF512 Contribute to Synthesis of Autoinducer Molecules and Nodulation of Phaseolus vulgaris.JOURNAL OF BACTERIOLOGY,Feb. 1998, p. 815–821.
2.Anne Edwards,1 Marijke Frederix,1 Florence Wisniewski-Dye´,2 Jacob Jones,1 Angeles Zorreguieta,3 and J. Allan Downie1*.The cin and rai Quorum-Sensing Regulatory Systems in Rhizobium leguminosarum Are Coordinated by ExpR
and CinS, a Small Regulatory Protein Coexpressed with CinI.JOURNAL OF BACTERIOLOGY, May 2009, p. 3059–3067
3.Juan E. Gonza´lez and Melanie M. Marketon. Quorum Sensing in Nitrogen-Fixing Rhizobia, MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2003,p. 574–592